(1.College of Physical Education,Liaoning Normal University,Dalian 116029,China; 2.Graduate School,Beijing Sports University,Beijing 100084,China)Abstract:In order to gain an insight into the effect of endurance training on the composition of heavy chain myosin (HCM) and the expression of myogenic regulating factors MyoD and Myogenin, and to probe into the relation be-tween the expression of MyoD and Myogenin and the conversion of HCM, the authors selected 20 male SD rats and randomly divided them into a resting control group (10 rats) and an endurance training group (10 rats), put the rats in the endurance training group on a treadmill with a + 10o slope for a running exercise at the speed of 20m/min once a day, 1 hour per time, 6 days a week, extracted the superficial layer of tibial anterior muscle of the rats in the two groups 28 days later, applied the SDS-PAGE method to measure the protein composition of HCM I, IIa, IIx and IIb, applied the RT-PCR method to measure HCMs and the expression of MyoD and Myogenin mRNA, and re-vealed the following findings: Endurance training did not change the protein expression of HCMs, after endurance training the expression of IIx – HCM mRNA was significantly reduced (P<0.05); endurance training lowered MyoD and Myogenin mRNA significantly, which indicate that endurance training reduce the gene expression of fast HCM, and that the reduction of fast HCM IIx may relate to the reduction of the expression of MyoD, and that MyoD and Myogenin may play a role in the adjustment of the expression of HCM. Key words:heavy chain myosin; myogenic regulating factor; endurance training |