| （1.School of Physical Education，South China Normal University，Guangzhou 510006，China；2.Peking Union Medical College，Institute of Medical Biology，Chinese Academy of Medical Sciences，Kunming 680118，China） Abstract: In order to observe the effects of exhausting exercise stress on the expression of the potassium ion chan-nels of T lymphocytes in the peripheral blood of mice and the regulatory volume decrease (RVD) of cells, and to probe into a possible mechanism of immunosuppression caused by exhausting exercise stress, the authors separated T lymphocytes from the peripheral blood after having duplicated the mouse exhausting model, measured its lym-phocyte transformation functions, revealed the functions of exhausting exercise stress in regulating the expression of Kv1.3 of T lymphocytes in the peripheral blood of mice from the change of the levels of genes and proteins respec-tively by using RT-PCR and Western blot, measured the change of T lymphocyte volume under a hypotonic condi-tion by using a cell imaging system, and revealed the following findings: exhausting exercise stress suppressed the functions of T lymphocytes of mice (P<0.05); the Kv1.3 mRNA and protein expression level of T lymphocytes in the peripheral blood of mice were regulated down significantly (P<0.05); exhausting exercise stress would affect the regulating ability of RVD. The said findings indicated that exhausting exercise stress caused the significant decrease of the KV1.3 mRNA and protein expression level of T lymphocytes in the peripheral blood of mice, and the weak-ening of the regulating ability of RVD, which suggested that the suppression of immune functions under an ex-hausting exercise stress condition may be related to the potassium channels.
Key words: exercise biochemistry；Kv1.3 immunosuppression；exhausting exercise stress |
