|（1.Sport Science Research Center，Beijing Sport University，Beijing 100084，China；2.Department of Anatomy，Beijing Sport University ，Beijing 100084，China）
Abstract: The authors intended to screen out molecular markers that predict the changing of hemogram indexes during the implementation of HiHiLo by analyzing the correlation between the polymorphism of the HIF-2a/EPAS1 gene and hemogram indexes before and after HiHiLo training. 1) the authors carried out 4-week HiHiLo training on 72 healthy testees, and measured hemogram indexes before and after the experiment; 2) the authors dissected the polymorphic loci of the HIF-2a/EPAS1 gene by applying the PCR-RFLP method; 3) the authors analyzed the corre-lation between the polymorphism of the HIF-2a/EPAS1 gene and hemogram indexes before and after HiHiLo train-ing, and probed into molecular genetic markers for evaluating hemogram effects during HiHiLo training. The au-thors revealed the following findings: 1)there was a significant difference between WBC changes in different geno-types (P<0.10), and there was a significant difference between M Retic# changes; 2)there was a significant differ-ence between MCV and MCH changes at the rs6715787 polymorphic locus in different genotypes (P<0.01, P<0.05). The said findings indicated the followings: 1)the rs7598371 polymorphic locus of the HIF-2a/EPAS1 gene is correlative with WBC and M Retic# before and after HiHiLo training, can be used as a molecular marker to pre-dict WBC and M Retic# changes during HiHiLo training; 2)the rs6715787 polymorphic locus of the HIF-2a/EPAS1 gene is correlative with MCV and MCH before and after HiHiLo training, can be used as a molecular marker to predict MCV and MCH changes during HiHiLo training.
Key words: sports biochemistry；HiHiLo；HIF-2a/EPAS1 gene；molecular marker；hemogram index