|（1.School of Sport and Health，Guangzhou University of Chinese Medicine，Guangzhou 510006，China；2.Chinese Resources and Research Center,Guangzhou University of Chinese Medicine，Guangzhou 510006，China）
Abstract: By using a continuous medium intensity horizontal running model, the authors studied the dynamic changes of the activity of anti-oxidation enzymes such as MDA, SOD, CAT and GSH-PX in the mitochondrion of skeletal muscle cells of rats taking in different dosages of antioxidant at different times, and inferred the lead factor that triggered the change of the level of stability of free radicals. The authors randomly divided 90 SD rats into a normal ginsenoside Rb1 group, a septuple ginsenoside Rb1 group, a physiological saline model group, and a physiological saline control group with 10 rats in each group in experiment 1, in which the test indexes were the SOD, MDA, CAT and GSH-PX in the mitochondrion of muscle cells, and into a control group, a medium intensity immediate group, a medium intensity 24h group, a medium intensity 48h group, and a medium intensity 72h group with 10 rats in each group in experiment 2. The author revealed the following finding: as compared with the physiological saline control group, the activity of antioxidant enzymes of rats in the normal ginsenoside group and the septuple ginsenoside group increased significantly, which suggested that during kinetic stressing the increase of the body’s anti-oxidation capacity was accompanied by the enhancement of the body’s anti-oxidation capacity, but the increase of the body’s anti-oxidation capacity under the stimulation free condition did not reversely trigger the reaction of the free radical stabilization system. The author further inferred that oxidation capacity is the lead factor for the horizontal movement of free radicals, while the enhancement of anti-oxidation capacity triggered exoge-nously has no negative feedback effect on the accumulation of free radicals.
Key words: sports biochemistry；kinetic stress；free radical；the same stable state；regulating factor