(Center for National Physical Fitness and Health,South China Normal University,Guangzhou 510006,China) Abstract: Purposes: To study the effects of long-term hyperthermic pretreatment on changes of the quantity of T-lymphocyte subsets, and to probe into whether long term hyperthermic pretreatment produces any effects on the immune functions of the human body. Experiment method: 10 male testees did an exhaustive exercise by applying the Bruce method before and after the one-month hyperthermic treatment (ambient temperature: 46oC, relative hu-midity: 90%); their elbow venous blood was drowned and their quantity of T-lymphocyte subsets was tested in a calm condition, immediately after exercising, 1h after exercising, and 24h after exercising. Experiment results: after long term hyperthermic pretreatment, in a calm condition, CD4+ increased and the difference was significant (P<0.05), while CD3+ and CD8+ increased and the differences were very significant (P<0.01); immediately after exercising, the differences in CD3+ and CD4+ were not significant (P>0.05), while CD8+ increased and the differ-ence was significant (P<0.05); the patterns of changing of CD4+ and CD8+ values were identical, and the differences were not significant (P>0.05); 24h after exercising, the differences in various indexes were all not significant (P>0.05). Result suggestions: after long term hyperthermic pretreatment, in a calm condition, the human body was still in an immunization stable state, having no immune function disorders, which suggests that long term hyper-thermic pretreatment plays a role in preventive protection of immunization of the human body; from immediately after exercising to 1h after exercising, the condition of immunosuppression of the human body was less serious than that before hyperthermic pretreatment. Key words: sports physiology;hyperthermic pretreatment;exhaustive exercising;T-lymphocyte subsets |