|（South China Normal University，Guangzhou 510006，China）
Abstract: To investigate the regulatory mechanism of thymocyte development during exercise-induced immuno-suppression, IL-7 and TGF-β1 as well as their mRNA of rats in thymus were tested. A total of 128 Sprague–Dawle male rats, aged 8 weeks, were divided into Control Group and Exercise Group. Incremental ergomitry Exercise pro-tocol (10 m•min-1 in week1, 20 min week2, 5 m•min-1 increased per week, successive 6 weeks, 6 d per week, 30 min per day). Samples were taken in week 0, week 2, week 4 and week 6 before exercise, just after exercise and 3 h after exercise, respectively. IL-7 and TGF-β1 as well as their mRNA of rats in thymus were calculated by ELISA and FQ-RT-PCR. Results:1) During six weeks incremental exercise: The responsive tendency of IL-7 and its mRNA presented the shape of “V” letter during week 0 and week2，while there was no distinguished changes during week4, and last decreased in week6. TGF-β1 and its mRNA presented that of inversed “V” letter during every week, except its mRNA was no prominent change in week0. Conclusion: The decrease of IL-7 and increase of TGF-β1 during Exercise-induced immunosuppression could be resulted the disorder of thymic microenvironment which effected thymocyte development.
Key words: exercise biochemistry；exercise-induced immunosuppression；interleukin-7(IL-7)；transforming growth factor beta 1(TGF-β1)；thymus；responsive characteristics