| （1.School of Physical Education，Weifang University，Weifang 261061，China；2.Liaoning Center of Football Administration，Shenyang 110081，China；3.School of Physical Education，Wenzhou Medical College，Wenzhou 325035，China） Abstract: In order to probe into the effects of acute exhaustive exercising on cell-free plasma DNA of teenage track and field athletes, and to seek for a new marker for athlete performance monitoring, the authors arranged 16 teenage track and field athletes to do an acute exhaustive exercise on a power treadmill, tested their Hematocrit (HCT), Cf-DNA, plasma malondialdehyde (MDA), creatine kinase (CK) and myoglobin (Mb) respectively before exercis-ing, immediately after exercising, at 15 min, 30 min, 1 h, 2 h, 3 h, 4 h, 12 h and 24 h after exercising, monitored the exercising process with a heat rate meter, recorded their exercising times and heart rates, calculated their exercising impulses (TRIMPS), and revealed the following findings: there was no significant change to HCT at all test times (P>0.05); cf-DNA increased significantly immediately after exercising (P<0.01), reached the peak value at 30 min (P<0.01), and lowered to the calm level at 4 h (P>0.05); plasma MDA, CK and Mb increased significantly immedi-ately after exercising (P<0.05, P<0.01, P<0.01), and maintained up to 24 h after exercising (P<0.01, P<0.05, P<0.01); TRIMPS was positively correlative significantly with the cf-DNA level at 15 min and 30 min after exer-cising (r=0.55, P<0.01 and r=0.64, P<0.05 respectively). The said findings indicated that cf-DNA is an early marker for tissue damage, can be used as a new index for athlete performance monitoring.
Key words: sports biochemistry；exhaustive exercise；cell-free plasma DNA；performance monitoring；athlete |
