| （1.School of Physical Education，Shaoguan University，Shaoguan 512005，China；2.Sport Biochemical Laboratory，Guangzhou Sport University，Guangzhou 510500，China） Abstract: The authors probed into the effects of hypoxic (13.6% oxygen content) training simulated under a normal pressure on brain tissues Ngb, HIF-1α, Bax and Bcl-2 of rats, so as to provide a theoretical reference for exercising and hypoxic adaptation. The authors divided 50 9 weeks old male SD rats randomly into a living low calm group, a living low training low group, a living high calm group, a living high training low group and a living high training high and low group; each group contained 10 rats. The rats in training groups were trained on a treadmill for 4 weeks at an intensity of 35m/min under a normal oxygen condition or 30m/min under a hypoxic condition, 1h/d (continuous exercising), 5d/week. After the experiment was finished, an ELISA kit was used to respectively measure the levels of brain tissues Ngb, HIF-1α, Bax and Bcl-2 of the rats, and the ratio of Bax to Bcl-2 was calculated. The authors revealed the following findings: 1) as compared with living low calm group, the HIF-1α, Bax and Bax/Bcl-2 of the rats in living low training low group increased (P<0.05 or P<0.01); the Ngb, HIF-1α, Bax and Bcl-2 of the rats in living high calm group, living high training low group and living high training and high and low group in-creased (P<0.05 or P<0.01); 2) as compared with living high calm group, the HIF-1α and Bcl-2 of the rats in living high calm group, living high training low group and living high training and high and low group increased (P<0.01); the Bax/Bcl-2 of the rats in living high training low group and living high training and high and low graoup de-creased (P<0.01); the Ngb of the rats in living high training and high and low group increased (P<0.01); 3) as com-pared with living high calm group, the HIF-1α and Bcl-2 of the rats in living high training low group and living high training and high and low group increased (P<0.05 or P<0.01); the Ngb of the rats in living high training and high and group increased (P<0.01), while their Bax/Bcl-2 decreased (P<0.05); 4) as compared with living high training low group, the Bcl-2 of the rats in living high training and high and low group increased (P<0.01); 5) Ngb expres-sion and HIF-1α expression showed a positive correlation (r=0.563, P<0.01); Ngb expression and Bax/Bcl-2 varia-tion showed a positive correlation (r=0.486, P<0.01); HIF-1α expression and Bax/Bcl-2 variation showed a positive correlation (r=0.353, P<0.05). The findings indicated the followings: training stimulation alone would cause the in-crease of brain tissue HIF-1 of rats; hypoxic stimulation alone would cause the increase of brain tissues Ngb and HIF-1α of rats; when such two factors as training and hypoxia were mutually combined, the increase of Ngb and HIF-1α would be more significant; the effects of living high training high and low on brain tissue Bcl-2 of rats were more significant than the effects of living high training low; the increase of Ngb and HIF-1α enabled Bax/Bcl-2 to develop in the direction favorable for neuron survival, which suggested that Ngb and HIF-1α had participated in the hypoxic tolerance and self protection of the central nervous system.
Key words: sports biochemistry；hypoxic training；neuroglobin；hypoxia induced factor-1α；apoptotic gene；rat |
