| （School of Physical Education，Zhengzhou University，Zhengzhou 450044，China） Abstract: In order to study the role played by CaMKII activated by exercising in adjusting the activity of combination of MEF2 and GLUT4 and the level of expression of gene GLUT4 in skeletal muscle of diabetic rats, so as to probe into any possible mechanism of exercising enhancing the expression of gene GLUT4 of diabetic rats, the author divided 100 healthy SD rats into a normal control group which contains 40 rats and a diabetic rat model establishment group which contains remaining 60 rats, 35 of which were successfully model established. Normal control rats and diabetic rats were randomly divided into 5 groups respectively by weight: a calm control group, a one-time exercising group, a one-time exercising+KN93 group, an endurance training group, an endurance+KN93 group; there were 10 groups totally. The treadmill speed was 20m/min; the exercising time was 1 h. The rats in the one-time exercising groups were sample taken 3h after one-time exercising. The rats in the endurance training groups were trained for 1 week using the same treadmill speed and exercising time, and sample taken 12 h after the last training. The author revealed the following findings: the activity of combination of MEF2 and GLUT4 inside skeletal muscle cells and the level of expression of gene GLUT4 in skeletal muscle of the rats in the diabetic rat endurance training group were all significantly lower than those of the rats in the normal control rat endurance training group, but significantly higher than those of the rats in the rats in the diabetic rat calm control group; the activity of combination of MEF2 and GLUT4 inside skeletal muscle cells of the rats in the endurance training + KN93 group was not significantly different from that of the rats in the calm con-trol group, but the level of expression of gene GLUT 4 in skeletal muscle of the rats in the endurance training + KN93 group was significantly higher than that of the rats in the calm control group. The findings indicate the followings: al-though exercising enhances the activity of combination of MEF2 and GLUT4 inside skeletal muscle by activating skeletal muscle CaMK II of diabetic rats, and the activity of combination of MEF2 and GLUT4 participates in regulat-ing the expression of gene GLUT4 in skeletal muscle of normal rats, it is not the only factor that affects the expression of gene GLUT4 in skeletal muscle of diabetic rats.
Key words: sports biochemistry；CaMKII；MEF2；GLUT4；diabetic rat |
