| （1.Key Laboratory of Adolescent Health Assessment and Exercise Intervention Ministry of Education，School of Physical Education and Health，East China Normal University，Shanghai 200241，China；2.School of Physical Education，Shanghai Normal University，Shanghai 200234，China) Abstract: In order to understand the effects of voluntary wheel exercise on spatial learning and memory and Notch signal pathway in hippocampus of C57BL/6 mice, the authors prepared 12 5-week old male C57BL/6 mice, ran-domly divided them into an exercise group (group R, n=6) and a control group (group C, n=6), let the mice in group R do a voluntary wheel exercise for 8 weeks, let the mice in group C live in a calm condition, starting from week 8, tested their learning and memory ability by utilizing Morris water maze, after the exercise was finished, cut the neck of the mice and took out hippocampus at both sides, measured the protein expression level of NICD in hippocampus of the mice by means of Western blot, measured the mRNA expression levels of Jagged-1, Notch-1, PS-1 and Hes-1 in hippocampus in the brains of the mice by means of RT-PCR, and revealed the following findings: the Morris water maze experiment showed that the learning and memory ability of the mice in group R was signifi-cantly stronger than that of the mice in group C; in Western blot test, the expression of NICD in hippocampus of the mice in group R increased significantly; in RT-PCR test, the mRNA expression levels of Jagged-1, Notch-1, PS-1 and Hes-1 in hippocampus of the mice in group R increased significantly as compared with those of the mice in group C. The said findings indicated that 8-week voluntary wheel exercise activated Notch signal pathway, en-hanced the spatial learning and memory ability of hippocampus.
Key words: sports biochemistry；learning and memory；Notch signal pathway；voluntary wheel exercise；mouse
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