| （1.Laboratory of Laser Sports Medicine，School of Physical Education and Sports Science，South China Normal University，Guangzhou 510006，China；2. School of Physical Education，Guangxi Normal University，Guilin 541004，China；3.School of Physical Education and Health，Zhaoqing University，Zhaoqing 526061，China) Abstract: In order to study the mesenchymal stem cells (MSCs) effect of carbohydrate supplementation for athletes by studying the effect of glucose in different concentrations on the proliferation of MSCs, the authors pretreated cell lines C3H10T1/2 of MSCs of mice with 5.0 and 22.5 mmol/L glucose respectively for 48 h, then continuously cul-tured them with glucose in different concentrations (5.0~500.0 mmol/L) or alternatively cultured them with 50.0~150.0 mmol/L and 22.5 mmol/L glucose for 12 h, measured the proliferation activity of 24~192 h cells by means of methylthiazolyldiphenyl-tetrazolium bromide/thiazolyl blue tetrazolium bromide (MTT) assay, and re-vealed the following findings: 1)the proliferation activity of the cells cultured with 22.5 and 50.0 mmol/L glucose was the best, and the glucose was called as normal glucose, while the glucose whose concentration was lower or higher than normal glucose was called as low glucose or high glucose; 2)when MSCs were pretreated with 22.5 mmol/L normal glucose and then continuously cultured with glucose in different concentrations, as compared with normal glucose, 100.0~300.0 mmol/L high glucose had a short-term proliferation promoting function; 3)longer-term high glucose culturing restrained cell proliferation. The said findings indicate that high glucose has a short-term pro-liferation promoting effect on MSCs pretreated with normal glucose.
Key words: sports biochemistry；mesenchymal stem cell；normal glucose；high glucose；mouse |
