| （1.School of Sport Science，Shanghai University of Sport，Shanghai 200438，China；2.Provincial Key Laboratory of Biochemistry，Guangzhou Sport University，Guangzhou 510500，China) Abstract: In order to probe into the effects of hypoxic exercise on PGC-1α in skeletal muscle of rats with alimen-tary obesity and its downstream factors, the authors built a model of SD rats with alimentary obesity induced by 7-week high-fat diet, then divided the rats randomly into a normoxic high-fat diet quit group (NHQ), a normoxic high-fat diet exercise group (NHE), a 16.3% hypoxic high-fat diet quit group (HGQ1), a 16.3% hypoxic high-fat diet exercise group (HGE1), a 13.3% hypoxic high-fat diet quit group (HGQ2), and a 13.3% hypoxic high-fat diet exercise group (HGE2), each of which consisted of 10 rats, continued to feed the rats with high-fat food, let them undergo 8-week endurance training, i.e. 20m/min, 40min/d, 5d/week, killed and sampled the rats 24h after the last exercise, measured 4 blood lipid indexes and blood glucose (BG) by means of test kit, measured PGC-1α and its downstream factors CPT-1, MCAD and PPARγ by means of qRT-PCR technology, and revealed the following find-ings: 1) 7-week high-fat diet could induced the increase or significant increase of the weight, BMI, as well as BG, CHO, LDL-L and TG contents of the rats (P<0.01 or P<0.05); 2) as compared with the rats in groups NHQ and HGQ1, the rats in groups HGE1, HGE2 and NHE had a decreased or significantly decreased weight (P<0.01 or P<0.05); as compared with the rats in group NHE, the rats in groups HGE1 and HGE2 had a decreased weight (P<0.05); 3) as compared with the rats in group NHQ, the rats in group NHE had a significantly increased MCAD mRNA expression (P<0.01); the rats in group HGE1 had increased or significantly increased PGC-1α, MCAD and PPARγ mRNA expressions (P<0.01 or P<0.05); the rats in group HGQ2 had a significantly increased PGC-1α mRNA expression (P<0.01); the rats in group HGE2 had increased or significantly increased PGC-1α , MCAD, CPT-1 and PPARγ mRNA expressions (P<0.01 or P<0.05); as compared with the rats in group NHE, the rats in groups HGE1 and HGQ2 had an increased PGC-1α mRNA expression (P<0.05); the rats in group HGE2 had in-creased or significantly increased PGC-1α , MCAD, CPT-1 and PPARγ mRNA expressions (P<0.01 or P<0.05); the rats in groups NGQ, HGQ1 and HGQ2 had a decreased or significantly decreased MCAD mRNA expression (P<0.01 or P<0.05); as compared with the rats in group HGQ1, the rats in groups HGE1 and HGQ2 had increased or significantly increased PGC-1α and MCAD expressions (P<0.01 or P<0.05); the rats in group HGE2 had signifi-cantly increased PGC-1α, MCAD and CPT-1 mRNA expressions (P<0.01); the rats in group NHE had increased or significantly increased MCAD and PPARγ mRNA expressions (P<0.01 or P<0.05). The said findings indicate the followings: 1) long-term high-fat diet can induce the occurrence of alimentary obesity; 2) hypoxia and/or endurance exercise can effectively control the weight of rats with alimentary obesity, increase PGC-1α in skeletal muscle and its downstream factors, while endurance exercise under a 13.3% hypoxic condition can achieve a better result.
Key words: sports biochemistry；hypoxic exercise；alimentary obesity；skeletal muscle；PGC-1α；rats |
